Localization and developmental regulation of a dispersed gene family-1 (DGF-1) protein in Trypanosoma cruzi
نویسندگان
چکیده
The abbreviations used are: ACN, acetonitrile; BSA, bovine serum albumin; DAPI, 4’,6’diamidino-2-phenylindole; DGF-1, dispersed gene family-1; DMEM, Dulbecco's Modified Eagle's Medium; dNTP, deoxynucleotide triphosphate; DTT, dithiothreitol; FITC-BSA, fluorescein isothiocyanate labelled bovine serum albumin; GST, glutathione S-transferase; HRP, horseradish peroxidase; IAA, Iodoacetamide; LIT, liver infusion tryptose; PCR, polymerase chain reaction; PBS, Dulbecco’s phosphate buffered saline; ORF, open reading frame; PPDK, pyruvate phosphate dikinase; RHS, retrotransposon hot spot; TcSCP, T. cruzi serine carboxypeptidase; TbVP1, T. brucei vacuolar proton pyrophosphatase; TFA, trifluoroacetic acid; TS, trans-sialidase; TRITC-Con A, tetramethylrhodamine isothiocyanate concanavalin A. Copyright © 2009, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Infect. Immun. doi:10.1128/IAI.00780-09 IAI Accepts, published online ahead of print on 19 October 2009
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Localization and developmental regulation of a dispersed gene family 1 protein in Trypanosoma cruzi.
The dispersed gene family 1 (DGF-1) is the fifth largest gene family in the Trypanosoma cruzi genome, with over 500 members (11). Many of the predicted DGF-1 protein products have several transmembrane domains and N-glycosylation and phosphorylation sites and were thought to localize in the plasma membrane. Here, we report that affinity-purified antibodies against a region of one of these prote...
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